![]() (C-D, C’-D’) distance traveled and center duration by open field testing. (A, A’) Repetitive behavior by marble burying test (A-E, A’-D’) Behavioral outcomes in offspring of colonized mice from 16 donors: Colonization with human ASD microbiomes reproduces behavioral deficits in mice. Differences in means were tested by linear mixed effects with donor diagnosis as a fixed effect and donor ID as a random effect.Ģ: Figure S2. (K) Aerobic and (L) anaerobic viable counts by plating on Tryptic Soy blood agar or Brucella blood agar, respectively. (J) Total 16S rRNA gene was measured by qPCR and quantified by a calibration curve with E. (J-L) Fecal bacterial load in offspring of colonized male mice. Differences in means were tested by linear mixed effects with donor diagnosis as a fixed effect and experimental stage, donor ID, and mouse sex as random effects. The fraction of taxa present in mice and respective donor, as well as the cumulative relative abundance of shared taxa in the donor are plotted. (H-I) Taxa engraftment in mice at the species level from 16S rRNA gene sequencing. (F-G) Taxonomic profile in donors (D), recipients (P), and offspring (F1) at the phylum level by diagnosis (F) and by donor (G) from 16S rRNA gene sequencing. ![]() Group differences by experimental stage (as in Panel C donors, recipients, and offspring) or by donor diagnosis were tested by pairwise PERMANOVA. (E) First two axes of a PCoA of unweighted UniFrac distances from 16S rRNA gene sequencing of human TD and ASD donor population. Group differences tested by Kruskal-Wallis test. (D) Box plots of α-diversity, as measured by the number of observed species from 16S rRNA gene sequencing. Differences between samples were tested by pairwise PERMANOVA test. (C) Box plots of pairwise distances of donor, recipients, and offspring mice to donor samples by unweighted UniFrac distances from 16S rRNA gene sequencing. Dark symbols denote samples that were further studied in depth. Group differences were tested by pairwise PERMANOVA. (B) First three axes of a principal coordinate analysis (PCoA) of unweighted UniFrac distances from TD (circles) and ASD (squares) donors from 16S rRNA gene sequencing. Sixteen samples used downstream are in dark-grey. Differences in means tested by Kruskal-Wallis. (A) α diversity as measured by observed amplicon sequence variants (ASVs) from 16S rRNA gene sequencing in TD and ASD individuals from which donor samples in this study were used. Engraftment fidelity for colonization of mice with human microbiomes. When the process is over, reboot the system.1: Figure S1.You have to wait to finish the scan process, choose the issues that were recognized, then select ‘Fix selected issues.’. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |